LOCUS       40924_49192        3526 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD036, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3526)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3526)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    389..394
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    392..412
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    413..418
                     /label=AgeI
                     /note="AgeI"
     regulatory      428..651
                     /note="PphoA_S7942; upstream region of phoA up to 25nt
                     upstream of the start codon in Synechococcus elongatus 
                     PCC7942"
                     /regulatory_class="promoter"
     regulatory      658..698
                     /note="RBS_LT; RBS and flanking sequences found in Life
                     Tech pENTR-SD/D Topo, start downstream of the att site and 
                     includes the T7 gene enhancer, RBS, TOPO binding site and 
                     directional TOPO overhang; Life Technologies; synthetic"
                     /regulatory_class="ribosome_binding_site"
     RBS             669..689
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             699..1412
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     protein_bind    complement(1435..1459)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1469..1496
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_feature    1505..1510
                     /label=XbaI
                     /note="XbaI"
     misc_feature    1511..1531
                     /note="G5C5; GC-adaptor for seamless assembly, flanking 
                     functional devices, custom sequences or cyanobacterial 
                     replicons; synthetic"
     misc_feature    1529..1534
                     /label=EcoRV
                     /note="EcoRV"
     rep_origin      complement(1779..2367)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2541..3398)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3399..3503)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag gcgcgccaga tatcccgccg ccgccgccgg ataccggttt
      421 cgaattggaa ttccgatgag acagataatc aacatcccat aaccagaaca gaattaatag
      481 gcagtatttt aaccagaatc tacttttgac ttaataattc agctcaatta ttcatcagca
      541 tgaatcagcg attgagcatt tttgctctta acaagaaatc ctacgagttt taactatttc
      601 ataatctatt ctcaatctcc tgtaaaccca agccgcctac cttggtttag gagatctccg
      661 cggccgcctt gtttaacttt aagaaggagc ccttcaccat gagtaaagga gaagaacttt
      721 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      781 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      841 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc gcgtatggtc
      901 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      961 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     1021 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     1081 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     1141 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     1201 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     1261 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     1321 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     1381 ggattacaca tggcatggat gaactataca aataataaaa gggtgggcgc gccgacccag
     1441 ctttcttgta caaactcggc cctgcaggag aaggccatcc tgacggatgg cctttttgaa
     1501 gctttctaga atcgggggcc cccgggggga tatcattaat taatggagca caagactggc
     1561 ctcatgggcc ttccgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1621 ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct cgctcactga
     1681 ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa
     1741 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1801 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1861 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1921 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1981 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2041 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2101 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2161 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2221 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2281 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2341 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2401 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2461 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2521 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2581 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2641 ggcttaccat ctggccccag tgctgcaatg ataccgcgag aaccacgctc accggctcca
     2701 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2761 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2821 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2881 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2941 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3001 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3061 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3121 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3181 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3241 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3301 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3361 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3421 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3481 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
